Plasmid_Backbone

Part:BBa_K4298008:Design

Designed by: Buyao Li   Group: iGEM22_Peking   (2022-09-29)


AcrB empV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3104
    Illegal SpeI site found at 908
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 908
    Illegal NotI site found at 915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2108
    Illegal XhoI site found at 2143
    Illegal XhoI site found at 4066
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3104
    Illegal SpeI site found at 908
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3104
    Illegal SpeI site found at 908
    Illegal NgoMIV site found at 2259
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4141


Design Notes

Cloned by PCR from pTac-RJN-AcrB-mCh with AcrB sequence deleted.